Acid Cellulase For Bio-polishing Sukacell P1000

Cellulose is an unbranched glucose polymer composed of anhydro-D-glucose units linked by 1, 4-b-D-glucoside bonds. These glycosidic bonds can be hydrolysed by cellulolytic enzymes. The native structure of the cellulose is composed of crystalline and less organized amorphous regions. SUKACell-P1000 is an enzyme preparation obtained from the submerged fermentation of a high cellulase-producing microbial strain. It is widely used in textile and garment stone wash industries.

WORKING MECHANISM

The 1, 4-b-D-glucosidic linkages in cellulose, lichenin and cereal b-D glucans are hydrolyzed to release free glucose units through the successive action of cellulase enzymes.

The less organized amorphous region at the center of the cellulose chain is initially attacked by endoglucanase by random cutting of the b-1, 4 glycosidic linkages within the chain thereby producing cello-oligosaccharides. Cellobiohydrolases I and II act on the cello-oligosaccharides from the reducing end and non-reducing end of the cello-oligosaccharides to release cellobiose. b- glucosidase act on the released cellobiose to produce b–D glucose units.

PROPERTIES

SUKACell-P1000 will function from a pH of 4.5 to 5.5; with pH 4.8 as an optimum. It will function from 45°C to 60°C and optimum temperature is 55°C.

Appearance: Cream to white powder (Note that color does not affect or reflect activity.)

Odor: Slight fermentation odor

pH (1% soln) 3.5-5.5

SPECIFICATION

SUKACell-P1000(activity is 4000 U/g)

High activity product is available upon request of our customers.

ENZYME ACTIVITY UNIT DEFINITION

Reducing sugar method At a pH of 4. 8 and temperature of 50 °C, to produce the amount of reducing sugar equivalent to 1 mg glucose, it needs l g solid enzyme (or 1mL liquid enzyme) and 1 h hydrolyze sodium carboxymethyl cellulose substrate, as 1 enzyme activity unit, state as u/g (or u/mL). CMCA – DNS for short.