Recombinant Carboxypeptidase B

1. DESCRIPTION
Carboxypeptidase B catalyzes the hydrolysis of the basic amino acids lysine, arginine, and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal-chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B . Recombinant carboxypeptidase B can replace native carboxypeptidase B for use in a variety of biotechnological processes.

2. MAIN FEATURES
Source Recombinant E. coli
Appearance White or off-white, or yellowish powder
Specific activity ≥170 units/mg pro.
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 33.8±3.4 kDa
UNIT DEFINITION： One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.

3. RECOMMEND USAGE
Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25 mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), and the optimum pH is pH 7.5-9.0.

4. STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in a sealed container.
It is stable within 24 months. After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃.
It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.

5. PRODUCT ADVANTAGE
① Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination, and any animal-origin material is not used in the production process.
② Stable quality: Mass production can ensure stable and continuous batch production. It is no difference between the batch and
the product quality is stable.
③ High purity: Higher specific activity other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host protein residues are less than the limits of biological products.
④ Lyophilized powder: The product is lyophilized powder and is convenient to store and transport.
⑤ Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
⑥ Complete quality documents: We can provide relevant regular support files according to customers’ requirements.

6.PRODUCT USAGE
The production of recombinant insulin and its analogues;
the quality test of recombinant antibodies;
the determination of C-terminal amino acids of proteins;
the production of other recombinant polypeptides;
and the enzymatic synthesis of certain specific compounds.